Journal: Diagnostic Pathology

Article Title: A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas

doi: 10.1186/1746-1596-8-58

Figure Lengend Snippet: Representative images of immunohistochemical results. Expression of calreticulin, prohibitin and HSP60 in adrenocortical carcinomas (ACC), adrenocortical adenomas (ACA) and normal tissues (N) ( A ). Calreticulin and prohibitin but not HSP60 were overexpressed in ACC compared with adrenocortical adenomas (ACA) and normal tissues ( B ).

Article Snippet: Frozen ACC and normal adjacent adrenocortical tissues were first homogenized using a sample grinding kit (GE Healthcare) with a lysis buffer (7 M urea, thiourea 2 M, 2% CHAPS, 18 mM DTT, 1 mM PMSF, 0.5% IPG buffer pH3~10NL), and then the extracts were centrifuged at 12,000 g, 4°C, for 1 hr.

Techniques: Immunohistochemical staining, Expressing

Journal: PLoS ONE

Article Title: N-octanoyl-Dopamine Is an Agonist at the Capsaicin Receptor TRPV1 and Mitigates Is Chemia-Induced Acute Kidney Injury in Rat

doi: 10.1371/journal.pone.0043525

Figure Lengend Snippet: A. Cryostat sections of rat renal tissue were stained for TRPV1 by indirect immunofluorescence using polyclonal an antibody (left). Note that distinct tubular structures were positive for TRPV1 (arrows). Omission of the primary was used as negative control (right). B. At higher magnifications it was observed that PGP9.5 positive nerve fibers (arrows) within the rat kidney also express the capsaicin receptor TRPV1 as shown by the overlay of both.

Article Snippet: TRPV1 in frozen kidney sections was detected using rabbit anti-TRPV1 polyclonal primary antibody (Alomone) and secondary anti-rabbit Cy3 (Jackson ImmunoResearch, PA, USA).

Techniques: Staining, Immunofluorescence, Negative Control

Journal: PLoS ONE

Article Title: N-octanoyl-Dopamine Is an Agonist at the Capsaicin Receptor TRPV1 and Mitigates Is Chemia-Induced Acute Kidney Injury in Rat

doi: 10.1371/journal.pone.0043525

Figure Lengend Snippet: A. Increases in free intracellular calcium in response to repetitive application of NOD displayed marked tachyphylaxis from stimulus to stimulus (grey trace). Application of the competitive TRPV1 antagonist capsazepine before and during the 2 nd stimulus (CPZ; black trace) abolished the NOD response. B. Mean change in intracellular calcium by NOD in cells challenged with (filled bars; n = 4) and without CPZ during the second stimulus (open bars; n = 5) indicated the specificity of NOD at TRPV1. The threshold for a significant response is indicated by the dotted line (*p<0.05, ***p<0.001 vehicle versus CPZ, student’s unpaired t-test).

Article Snippet: TRPV1 in frozen kidney sections was detected using rabbit anti-TRPV1 polyclonal primary antibody (Alomone) and secondary anti-rabbit Cy3 (Jackson ImmunoResearch, PA, USA).

Techniques:

Journal: PLoS ONE

Article Title: N-octanoyl-Dopamine Is an Agonist at the Capsaicin Receptor TRPV1 and Mitigates Is Chemia-Induced Acute Kidney Injury in Rat

doi: 10.1371/journal.pone.0043525

Figure Lengend Snippet: A. Human PTECs were marked with DAPI and stained with a primary anti-TRPV1 antibody (giunea-pig polyclonal serum); omission of a primary antibody served as negative control ( B ). C. In addition TRPV1 was assessed in lysates of unstimulated (1) or TNF-α stimulated PTECs (2) by Western blotting. TRPV1 transfected HEK cells (3) were used as positive control to indicate the molecular weight of TRPV1.

Article Snippet: TRPV1 in frozen kidney sections was detected using rabbit anti-TRPV1 polyclonal primary antibody (Alomone) and secondary anti-rabbit Cy3 (Jackson ImmunoResearch, PA, USA).

Techniques: Staining, Negative Control, Western Blot, Transfection, Positive Control, Molecular Weight